Break it down first. You didn't give a lot to go on so my advice is similarly disorganized.
What do you know about the physical setup? Which port? What's on the other side of the PLC? Which one is the master/slave? Is it intermittent issue, is it limited to certain register vs all of them?
There are tools you can download for free, the 1st hit on google with "modbus slave simulator" is decent. Install it on your PC to make it the slave and see if the master device is functioning properly then go from there.
I should add that typically in a SCADA environment, the RTU is usually the master and the PLC is the slave but it doesn't have to be that way.