If you make the ACC negative you will fault the processor. Since a timer is not a device, it is a computer instruction, it calculates the elapsed time from a reference time that is stored in the least significant byte of T4:X.0 and so it can only count up.
You can compute the amount of time remaining by subtracting the ACC from the PRE and storing the value in an N word. The value stored in the N word will count down from the preset to zero.