So now you can look at the FCs online.
In each Ladder line with an output you can follow the logical flow while ou are online (yes use the "glasses").
If the output coil in the ladder rung goes green the output should go on.
edit: If there is a mistake so that the FC is actually not called, then the rung wont go green.
If the output does not go on despite coil in the ladder rung is green:
1. Have you use the output twice, so that in one rung it is turned on and in a following rung it is turned off ?
2. Have you used the correct address ?
3. Is there power supply to the output ? Refer to the electrical documentation for the particular PLC model you are using.