Both lines have a XIC that shows Q100.00 is OFF, even though the output on the rung says it's ON.
The way scanning of the program works it is possible that when Rung 0 is scanned the line is not true and the output is off, which on the next rung means the timer would not run.
Then later in the program another rung turns the output ON and when the between-scan communications happens with the online software it shows ON, but still wasn't for that rung.
That said, if this is a 2 line program for a test, with no other rungs, then it might be the PLC is not in Run mode. In Program mode the inputs will show the status if On or Off, but timers and outputs will not be turned on.
I just noticed your timer is set to 0 BCD, that is 0.0 seconds, maybe 0.00 depending on the PLC.. For 50 seconds that should be 500 or 5000 BDC.